小鼠细胞因子抗体芯片3
更新 ：2014/7/21 17:06:06 点击 ：1899
- 产品型号 AAM-CYT-3
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
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|
Axl
|
BLC
(CXCL13) |
CD30 Ligand
(TNFSF8) |
CD30
(TNFRSF8) |
CD40
(TNFRSF5) |
|
CRG-2
|
CTACK
(CCL27) |
CXCL16
|
Eotaxin-1
(CCL11) |
Eotaxin-2
(MPIF-2/CCL24) |
|
Fas Ligand
(TNFSF6) |
Fractalkine
(CX3CL1) |
GCSF
|
GM-CSF
|
IFN-gamma
|
|
IGFBP-3
|
IGFBP-5
|
IGFBP-6
|
IL-1 beta
(IL-1 F2) |
IL-10
|
|
IL-12 p40/p70
|
IL-12 p70
|
IL-13
|
IL-17A
|
IL-1 alpha
(IL-1 F1) |
|
IL-2
|
IL-3
|
IL-3 R beta
|
IL-4
|
IL-5
|
|
IL-6
|
IL-9
|
KC
(CXCL1) |
Leptin
|
Leptin R
|
|
LIX
|
L-Selectin
(CD62L) |
Lymphotactin
(XCL1) |
MCP-1
(CCL2) |
MCP-5
|
|
M-CSF
|
MIG
(CXCL9) |
MIP-1 alpha
(CCL3) |
MIP-1 gamma
|
MIP-2
|
|
MIP-3 beta
(CCL19) |
MIP-3 alpha
(CCL20) |
Platelet Factor 4
(CXCL4) |
P-Selectin
|
RANTES
(CCL5) |
|
SCF
|
SDF-1 alpha
(CXCL12 alpha) |
TNF RI
(TNFRSF1A) |
TNF RII
(TNFRSF1B) |
TARC
(CCL17) |
|
I-309
(TCA-3/CCL1) |
TECK
(CCL25) |
TIMP-1
|
TNF alpha
|
Thrombopoietin
(TPO) |
|
VCAM-1
(CD106) |
VEGF-A
|
|||
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
62
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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